An integrative urinary metabolomic study of the therapeutic effect of Guizhi Fuling capsule on primary dysmenorrheal rats based 1H NMR and UPLC–MS

Nov 27, 2018Journal of pharmaceutical and biomedical analysis

Urine metabolite changes linked to Guizhi Fuling capsule treatment in rats with primary menstrual pain

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Abstract

A total of 36 potential biomarkers were identified that may be involved in metabolic pathways related to primary dysmenorrhea.

Primary dysmenorrhea (PD) is associated with disruptions in various metabolic pathways, including the TCA cycle and lipid metabolism.
Guizhi Fuling capsule (GFC) may exert therapeutic effects on PD by regulating multiple metabolic processes.
The study utilized advanced techniques to analyze both non-polar and polar metabolites in urine.
Identified biomarkers could play a role in the early diagnosis and prediction of primary dysmenorrhea.

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Guizhi Fuling capsule (GFC) was an important traditional Chinese herbal medicine used for the treatment of primary dysmenorrheal (PD). The aim of this study was to evaluate the anti-dysmenorrheal effect of GFC on dysmenorrheal rats induced by oxytocin and to investigate its mechanism of action. An integrative urinary metabolomic study based onH NMR and UPLC-MS was used to investigate the therapeutic effect of GFC on PD rats. In addition, in order to obtain more potential biomarkers and to investigate the global urine metabolic profile associated with PD, we combined the characteristics of RP-UPLC-MS with HILIC-UPLC-MS on metabolomic platform to find non-polar and polar metabolites. Finally, a total of 36 potential biomarkers were identified as being primarily involved in the TCA cycle, gluconeogenesis, glycolysis, pentose phosphate pathway, lipid metabolism, energy metabolism, amino acid metabolism and intestinal flora metabolism, and PD could influence the balance of many of these metabolic pathways in vivo. Furthermore, these results also suggested that the GFC had therapeutic effects on PD rats via the regulation of multiple metabolic pathways. In conclusion, the variations in potential biomarkers revealed the therapeutic mechanism of GFC, and these potential biomarkers were both significant for early diagnosis and predicting PD. 1

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An integrative urinary metabolomic study of the therapeutic effect of Guizhi Fuling capsule on primary dysmenorrheal rats based 1H NMR and UPLC–MS

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