Gut microbial dysbiosis is associated with metabolism and immune factors in liver fibrosis mice

Dec 31, 2023International journal of biological macromolecules

Imbalanced gut bacteria linked to metabolism and immune changes in mice with liver scarring

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Abstract

CCl4 treatment resulted in a significant increase in Desulfobactera abundance in the gut microbiota of liver fibrosis mice.

CCl4 disrupted the balance of gut microbiota, particularly affecting the genera Enterorhabdus and Desulfovibrio.
A total of 1416 genes were identified as differentially expressed in the liver tissue of CCl4-treated mice compared to controls.
The differentially expressed genes were primarily associated with lipid metabolism and immune system processes.
The abundance of certain gut microbiota phyla and genera showed negative correlations with metabolism-related genes.
Conversely, these microbiota were positively correlated with immune-related genes and those involved in the PI3K-Akt signaling pathway.

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Gut microbial dysbiosis has always served as a potential factor in the occurrence and development of liver fibrosis. Liver and gut microflora can regulate each other through the gut-liver axis. In this study, the 16S rRNA and RNA-seq were chosen to sequence gut microbiota alteration and liver differentially expressed genes (DEGs) in carbon tetrachloride (CCl4) included-liver fibrosis mice, and analyze the correlations between gut microbiota constituents and DEGs. Results indicated that, CCl4 significantly increased the abundance of Desulfobactera in the phylum level, destroyed gut microbiota balance in the genus levels, especially Enterorhabdus and Desulfovibrio. Through analysis, 1416 genes were found differentially expressed in mice liver tissue in the CCl4 Group, compared with the Control Group; and the DEGs were mainly involved in the lipid metabolic process and immune system process. The correlation analysis revealed that the relative abundance of microbiota phylum (Desulfobactera) and genus (Enterorhabdus and Desulfovibrio) was negatively correlated with the metabolism related genes, while positively correlated with immune-related genes and the genes enriched in PI3K-Akt signaling pathway. To sum up, CCl4 can partially regulate gene expression in metabolism, immune response and the PI3K/Akt pathway, and further maintain the stability of the gut environment in liver fibrosis mice.

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Gut microbial dysbiosis is associated with metabolism and immune factors in liver fibrosis mice

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