The Gut Microbiome's Role in Brain and Mental Health Disorders

The human GM is estimated to consist of 10–100 trillion microorganisms together with the genes of their cells. The totality of this genome is estimated at 3.3 million genes, compared to approximately 22,000 genes in the human body [3,38,39]. There is about 99.9% identity of the human genome, but there may be 70–90% variation among the microorganisms’ genomes of different individuals [15,40,41,42,43]. The genetic complement of the GM, or in other words, the set of “microorganisms (eukaryotic archaea, bacteria and viruses), the genetic information they carry and the environment in which they interact”, is believed to exceed that of the human genome by 100 times [4,44,45]. Until recently, the main method of isolating and recording the microorganisms of the GM was their culture [46,47,48,49,50]. the various bacteria are classified into Gram positive and Gram negative, depending on the ability of their cell wall to retain the pigment or not after treatment with alcohol [51,52,53]. The GM remains relatively stable throughout life, with average changes. Its basic constitution is formed in the early years [54,55]. The commonly accepted theory so far is that the gastrointestinal system of the newborn is sterile, but also, several studies found that the microorganisms pass to the fetus through the placenta, umbilical cord, and amniotic fluid, but this remains under debate [56,57,58]. In the first months of life, anaerobic microorganisms predominate, mainly, Bacteroides, Bifidobacterium, and species from the Lactobacillaceae family, which are classified in the phyla Bacteroidota, Bacillota, and Actinomycetota, respectively. Pseudomonadota is also added from breast milk. Studies show that approximately 28% of bacteria in infants comes from breast milk. Later, with the introduction of other different aliments (from ages 5 months to 2 years), bacteria of the Ruminococcaceae and Lachnospiraceae families, which belong to the Bacillota phyla, also appear in the GM. Around the age of 2 or 3 years, strains of the Verrucomicrobia phylum are also found. From around the age of 5, the GM of children begins to resemble that of adults (Figure 1 and Figure 2) [59,60,61,62].

Studies have shown that the GM of adolescents still has some differences compared to that of adults. These differences are found at the level of the bacterial genus and mainly concern Bifidobacterium, which appears at almost twice the percentage compared to adults (Figure 3). Credits: Original figure by I.A. Charitos [63,64,65].

In adults, the main phyla of the GM are Bacillota and Bacteroidota, which account for 90% of the GM. Actinomycetota, Pseudomonadota, Fusobacteriota, and Verrucomicrobiota are found in smaller percentages. The genus Clostridium accounts for 95% of the Bacillota and the remainder is represented by the genera Bacillus, Enterococcus, and Ruminococcus and the Lactobacillaceae family. Bacteroidetes consists mainly of Bacteroides and Prevotella, while the main genus of Actinomycetota is the Bifidobacterium. Everyone’s GM displays different clusters of microorganisms that shape everyone’s intestinal microbiota type. The enterotype is specific and stable for each person throughout his life, and in the case of change, it returns to the original composition. The most important difference between these types lies in the way energy is produced from the available elements of the intestinal content. The intestine types seem to be determined by eating habits, mainly during childhood age (Figure 4) [66,67,68,69].

Several factors influence the composition of the GM. In neonates and infants, gestational age at birth plays a significant role. Premature birth results in delayed intestinal colonization, with limited microbiome diversity, reduced Bifidobacterium and Bacteroides, and an increase in potentially pathogenic bacteria such as Enterobacteriaceae. Breastfeeding is also affected by prematurity, impacting the composition of the GM [70,71,72]. The mode of birth further influences GM. During natural labor, the newborn is colonized by maternal microbiota including Prevotella, Sneathia, Bifidobacterium longum, and others, alongside organisms such as Escherichia coli, Staphylococcus, Bacteroides fragilis, and Streptococcus. In contrast, cesarean-section births lead to colonization by hospital-associated microorganisms like Staphylococcus and Corynebacterium, with reduced diversity and the absence of Escherichia, Shigella, and Bacteroides [73,74,75,76]. Diet and feeding mode also significantly affect the GM composition. Breastfeeding promotes healthier GM, characterized by increased Bifidobacterium spp. and decreased C. difficile and E. coli. The quality of breast milk is influenced by maternal gut health during pregnancy [77,78]. Age-related changes in diet lead to variations in GM. Adults over 70, for example, experience changes linked to dietary monotony and inflammatory conditions. Furthermore, geographic and cultural differences in diet contribute to GM variations, with diet being a key regulator of microbiota composition. Specific bacteria, including Latilactobacillus sakei and Faecalibacterium prausnitzii, are affected by diet, and obesity and BMI also play a crucial role in altering the GM composition, particularly in children. Obese children show increased Bacillota and decreased Bacteroidota, while variations in BMI result in microbiota differences [79,80,81,82,83,84]. In cases of conditions such as anorexia nervosa, significant changes in GM occur, with an increase in Enterobacteriaceae and Methanobrevibacter smithii, and a decrease in Roseburia, Ruminococcus, and Clostridium [85,86,87]. Furthermore, the use of antibiotics and xenobiotics, and drug abuse, has a substantial impact on GM. The type of antibiotic, dosage, and administration period affect microbiota composition. Macrolides, for example, decrease Actinomycetota and increase Bacteroides and Bacillota, while Penicillin and Clarithromycin have similar, though less severe, effects. The misuse of antibiotics, particularly in early childhood, has been linked to reduced microbiota diversity and the development of allergic diseases such as asthma and food allergies, largely influenced by specific strains of the Clostridia genus [88,89,90,91,92,93].

Furthermore, it has been proven that negative changes (dysbiosis) in the composition of the GM (are related also to several pathological conditions [94,95,96,97]. As we mentioned, deviations from a healthy lifestyle directly affect the GM and are related to many metabolic diseases (unhealthy gut metabolome), which, in turn, can lead to an exacerbation of symptoms or new-onset neurocognitive disorders. It must be mentioned that the first association of various diseases with the intestine is attributed to the father of medicine Hippocrates [94,98,99,100,101].

Until recently, studies on the GBA focused on digestion and satiety. In recent years, however, emphasis has been placed on the degree of influence of higher functions, psychological state, and behavior by GBA communication [102,103,104,105]. Several research efforts are constantly being made in the direction of correlating various brain functions with GM. Animal studies more directly indicate the GBA communication [106,107,108,109,110]. The GM–brain axis includes the central nervous system (CNS), the autonomic nervous system (ANS), the enteric nervous system (ENS), the hypothalamic–pituitary–adrenal axis (HPA), and their neurotransmitters. The ANS collects information from the ENS and, through its sympathetic and parasympathetic branches, conveys it to the CNS. Conversely, it carries impulses from the CNS to the periphery. The HPA axis functions as a key regulator of the body’s response to various stressful situations [111]. The ENS is connected to the ANS, but also to the peripheral nervous system (PNS), and controls the behavior of the gastrointestinal tract, specifically, its motility, local blood flow, and absorbency, but also the secretions of the intestinal mucosa, and regulates endocrine and immune function [112,113]. Enteric neurons are connected to the ANS but do not have a direct neuronal connection with the CNS. They are organized in microcircuits, which receive stimuli from local factors and can respond to them directly, without the mediation of the CNS. Nevertheless, there is usually communication between the two systems—through neural circuits, which initially connect the ENS to the ANS and, indirectly, to the CNS—and one affects the other [114,115,116]. The ANS consists of sympathetic and parasympathetic nervous systems. The sympathetic nervous system is related to the control of gut motility, intestinal mucosal secretions, and vascular regulation by depressing the ENS and through the direct vasoconstriction of his action. The main neurotransmitters of the sympathetic nervous system are, in addition to noradrenaline (NE), neuropeptide Y (NPY) and ATP. The parasympathetic nervous system is mainly represented by the pneumogastric (or vagus) nerve [117,118,119,120]. The pneumogastric nerve is the 10th cranial nerve and its action on the gastrointestinal tract is related to food intake, digestion, the maintenance of the gastrointestinal barrier, and immunity. More specifically, the endings of the pneumogastric nerve in the intestinal mucosa act as chemoreceptors and are activated by hormones, peptides, and other elements, which release the endothelial and neuroendocrine cells of the intestine [121,122,123]. At the same time, the mechanoreceptors of the pneumogastric nerve sense the distension of the gastrointestinal tract. The result of these stimuli is causing an increase in the secretion of gastric fluids, insulin, and glucagon, with an effect on the digestion process. The pneumogastric nerve contributes to the maintenance of the intestinal barrier mainly by activating the mucus cells in the intestinal wall and strengthening the connections between them [124,125]. Finally, inflammatory mediators such as cytokines and chemical mediators from mast cells, but also several pathogens, activate the pneumogastric nerve. The result is the activation of the cholinergic anti-inflammatory pathway, in which stimuli carried by the pneumogastric nerve, mediated by acetylcholine, regulate the action of macrophages around inflammation [126,127,128]. The HPA axis is part of the limbic system, which is associated with emotion and memory. The HPA axis, with its complexity of endocrine pathways, plays a regulatory role in the response to stressful situations, but also in metabolism, immune responses, and the ANS [129,130]. In response to stressful situations, the hypothalamus secretes adrenocorticotrophic hormone-releasing factor (CRF), which causes the pituitary to release adrenocorticotropin-releasing hormone (ACTH), which leads to the secretion of cortisol from the adrenal glands [131]. Glucocorticoids that enter the circulation mobilize energy reserves, activate lipolysis and, through the mediation of the ANS, lead to vasoconstriction, and are, therefore, a consequence of the response to the stress-inducing stimulus and, therefore, to the psychological state (Figure 5) [129,132].

An important role in the GBA connection is also played by a multitude of chemicals, which act as neurotransmitters. Many of these neurotransmitters are, in turn, linked to the GMe. Among the most important neurotransmitters are catecholamines (adrenaline, noradrenaline, and dopamine), serotonin, γ-aminobutyric acid (GABA) and adenosine triphosphatase (ATP), and histamine [133,134]. Serotonin is synthesized in the midbrain, specifically, in its nucleus seam. Recent research, however, has shown that some of it is also synthesized in intestinal nerves along the intestinal tract. Serotonin’s precursor is tryptophan, an amino acid derived from proteins that are consumed in foods such as meat, dairy, and fruit. Serotonin then binds to its various receptors with the corresponding effect [135]. Certain GM bacterial species, such as E. coli, are thought to be able to use tryptophan as well, indirectly reducing serotonin levels [136,137]. Besides serotonin, tryptophan is also a precursor of kynurenic acid. It seems that GM also plays a role in determining the direction that the metabolism of tryptophan will take, thus influencing the adequacy or lack of serotonin in the body [138]. Serotonin’s relationship with the gut is also indirectly demonstrated by the fact that drugs such as selective serotonin reuptake inhibitors, in addition to their action on the CNS, also affect several symptoms of chronic diseases of the gastrointestinal tract [139,140]. The basic precursor of all catecholamines is the amino acid L-tyrosine, which is first converted into L-dopa by the enzyme tyrosine hydroxylase and then into dopamine. The main regulator of the action of tyrosine hydroxylase is endogenous neuropeptide Y (NPY). Dopamine is the first catecholamine in the production chain and is mainly synthesized in the CNS [141,142]. Adrenaline and noradrenaline are synthesized either locally in the postganglionic nerve fibers of the sympathetic system or centrally in the adrenal glands. Each of the catecholamines binds to specific receptors. Noradrenaline decreases blood flow to the gut and adrenaline increases it. The action of dopamine depends on its concentration, as in low doses, it causes vasodilation, and in high doses, it causes vasoconstriction [143,144]. The participation of catecholamines in the regulation of gut motility is expressed by its reduction by adrenaline and, conversely, its increase by noradrenaline. In addition to the perfusion and motility of the intestine, adrenaline and noradrenaline also affect the absorption of nutrients [145,146]. Dopamine, on the other hand, seems to be less involved in the absorption of various substances, but is related to the reward and satisfaction system after digestion. Studies show that dopamine’s involvement in the reward system is directly regulated by stimuli from the gastrointestinal tract. New evidence shows that catecholamines are also involved in the immune mechanism of the gut, but not in the expected way. It seems that these substances reduce the immune response of the intestinal mucosa and increase the “aggressiveness” of different bacteria [147]. Adenosine triphosphatase (ATP), in addition to being a basic source of energy, also has a role as a neurotransmitter. Although its relationship to the GBA is not clear, its receptors have been identified along the intestinal tract, and in areas of gut inflammation, the concentration of ATP is increased. GABA is the main inhibitory neurotransmitter of the CNS. In the gastrointestinal tract, however, the action of GABA is reversed as it mainly causes stimulation. Its action is mainly related to intestinal peristalsis and immunity [148,149].

Several studies conclude that GM bacteria, in addition to influencing neurotransmitters and their precursors, also secrete factors with a similar effect. For example, members of the genera Candida, Streptococcus, Escherichia, and Enterococcus have been shown to secrete serotonin, members of Escherichia, Bacillus, and Saccharomyces produce dopamine and noradrenaline, members of the genus Lactobacillus produce acetylcholine, and Bifidobacterium synthesizes GABA [150,151]. In addition to the above, the bacteria of the GM also secrete several other substances. Some of these substances, although not neurotransmitters in the strict sense of the term, act in a similar way to the nervous system. Among these substances are those from the bacteria metabolism such as short-chain fatty acids (SCFAs), with butyric, propionic, and acetic acid as main representatives, which have a particularly important position [152,153]. The SCFAs have a protective local effect on the intestine, as they contribute to its integrity, mucus production, and intestinal immunity. The blood–brain barrier is crossed by SCFAs; moreover, they are isolated in the cerebrospinal fluid, exerting a neuroprotective effect, and are possibly involved in neurodevelopmental and neurodegenerative processes [152,154].

For example, propionic acid is produced mainly by Bacteroidota, Bacillota, and Lachnospiraceae, while butyric acid is produced by Faecalibacterium prausnitzii, Eubacterium rectale, Eubacterium hallii, Coprococcus comes, and Coprococcus eutactus. In conclusion, the function of the GM–brain axis appears to be based on four mechanisms. The first is the impulses carried from the periphery to the brain via the pneumogastric nerve and spinal nerves. The following is the stimuli that are conveyed through cytokines and the various 23 types of hormones. The last mechanism is the action of the derivatives of the GM microorganisms themselves, either directly in the brain through the blood circulation, or indirectly by affecting intermediate mechanisms [155,156,157].

In recent years, the studies that associate disturbances in the intestinal microbiome and, by extension, in the gut–brain axis, with various pathological conditions are increasing. Relatively recently, interest has also shifted towards neurological, psychiatric, and neurodevelopmental disorders, which are possibly influenced by variations in the GM [158]. Suspicion of the influence of the GBA on psychiatric diseases has been around for quite some time. The relationship between stress and the GMe is bidirectional [159]. Studies have shown that stress can alter GM through the activation of the HPA and the autonomic nervous system (ANS). Conversely, it is now proven that changes in GM can cause or limit stress in the body [160,161]. Most relevant studies have been carried out in animal models. For example, it has been shown that in germ-free rodents, the addition of a specific species of Bifidobacteria led to increased activation of the stress-relevant stress axis. The introduction of Lactiplantibacillus plantarum had the opposite effect by reducing anxiety [162,163]. Parkinson’s disease is another neurological condition that is beginning to be associated with the GM composition. It is a neurodegenerative disease mainly characterized by the loss of dopaminergic neurons in the substantia nigra of the brain, accompanied by the accumulation of α-synuclein in the neurons [164,165,166]. There is now evidence to support the appearance of α-synucleinopathy (αS-pathy) already in the initial stages of the disease in the ENS [167]. Patients experience gastrointestinal disturbances due to increased intestinal permeability due to the reduced concentration of SCFAs. The composition of the GM in patients with Parkinson’s disease shows significant differences. Specifically, there is an increase mainly in Bifidobacterium, Akkermansia, Oscillibacter, Alistipes, and the Lactobacillaceae family and a decrease in Roseburia, Fusicatenibacter, and Faecalibacterium, which produce butyric acid and have an anti-inflammatory effect. The association of Parkinson’s disease with changes in the GM paves the way for studies in other alpha-synucleinopathies such as Alzheimer’s disease [168,169,170]. Multiple sclerosis is an inflammatory disease of the CNS of immune etiology. Of the neurological diseases that have been associated to some extent with GM, some indicative ones are mentioned [171]. Comparisons of GM in multiple sclerosis patients and healthy individuals show differences not so much in qualitative as in quantitative composition, with some populations of microorganisms appearing in greater or lesser numbers. More specifically, the population of Akkermansia muciniphila appears proportionally increased in patients with multiple sclerosis in recent research. The Akkermansia has been linked to inflammatory pathways and the activation of the complement system [172]. This connection of multiple sclerosis with the composition of the GM has opened new therapeutic avenues and related research is constantly being carried out [173]. The link between depression and bowel disorders has been identified for quite some time, although the exact mechanism has not yet been confirmed. Among the pathophysiological mechanisms that have been proposed is the one related to permeability of the intestinal mucosa. According to this mechanism, stressful situations affect the intestinal epithelial barrier, resulting in an increase in its permeability [174].

This allows Gram-negative bacteria to meet the ENS and immune cells. An immune response is induced with accumulation of inflammation factors. Accumulated cytokines lead to the stimulation of the pneumogastric nerve, which, in turn, activates the HPA axis, resulting in mood disorders [175,176,177,178]. At the same time, in patients with depression, a decrease in the diversity and differentiation of the GM and a predominance of Bacillota, Bacteroidota, and Actinomycetota [179,180,181] has been observed. Schizophrenia and the relationship with GM disturbances is a relatively new field of research. Inflammatory processes have been shown to be involved in the pathophysiology of the disease [182,183,184]. Disturbances in GM may alter the permeability of the intestinal wall and, thus, contribute to inflammation, through the action of cytokines [98,185]. At the same time, bacterial species that produce butyric acid have been identified in reduced concentrations in schizophrenia. Finally, the confirmed action of GM derivatives on the hematological barrier can explain the enhancement of CNS inflammation in disorders of its composition [186]. The existence of an inflammatory process of the CNS in schizophrenia has now been proven. Specific changes in the GM, such as a reduction in α-diversity markers, have been documented in patients with schizophrenia. Bacteria such as Veillonellaceae and Lachnospiraceae have been associated with disease severity. Differences in GM composition have also been identified in schizophrenia patients before and after receiving treatment [187]. Bipolar disorder affects approximately 40 million people worldwide and is mainly expressed by mood swings [188,189]. The relationship of the disease with gastrointestinal disorders such as irritable bowels has long been proven. Patients with bipolar disorder show evidence of peripheral inflammation with an increase in cytokines during disease flares. Also, inflammatory processes seem to coexist with changes in tryptophan metabolism in these patients. These changes, as already mentioned, are also related to the action of the GM [190]. More specifically, there is a decrease in the populations of microorganisms such as Faecalibacterium, and an increase in the populations of the Lactobacillaceae family and Enterococcus, as well as Actinomycetota phyla. At the same time, the pharmaceutical preparations used to treat bipolar disorder have also been shown to affect the balance between the individual populations of GM. In this way, the relationship between the change in the composition of the GM and the manifestation of bipolar disorder is indirectly demonstrated [191].

The neurodevelopmental disorders include ASD, intellectual disability, attention deficit hyperactivity disorder (ADHD), communication disorders, developmental motor disorders including tics, and specific learning disabilities [192]. The most sensitive periods for the appearance of neurodevelopmental disorders are considered the fetal period, childbirth, the breastfeeding period, and, in general infancy. During this period, factors such as stress, eating habits, and the mother’s endocrinological and immune status, but also the composition of the mother’s gut and vaginal microbiota, as well as the route of delivery, can affect the development of the child’s CNS, leading or not to the appearance of neurodevelopmental disorders [193,194]. In ADHD, hypermobility with changes in the composition of the GM appeared in the literature much more recently, and mainly after the corresponding studies concerning autism spectrum disorders. Most of these theories are since several conditions associated with the onset of ADHD in a person are also associated with changes in the GM. Given the function of the GBA, as already mentioned, a theoretical etiological model for ADHD is created [195,196,197]. More specifically, the relationship between the mode of birth and ADHD has already been suggested through the increased rates of its occurrence in people born by the caesarean section. Research indicates that children born by caesarean section may have a higher risk of developing ADHD compared to those born via vaginal delivery. The mode of birth affects the initial colonization of the newborn’s gut microbiota, which plays a crucial role in neurodevelopment [198,199,200]. Infants are exposed to different microbial environments than those born vaginally, potentially impacting the gut–brain axis. Additionally, the stress response during vaginal delivery triggers the release of hormones that aid brain development, a process that might be less pronounced in caesarean section births. Perinatal factors, such as complications during labor that necessitate emergency CS, are also linked to an increased risk of ADHD. While there is evidence suggesting a link between caesarean section and ADHD, the relationship is complex and influenced by multiple factors, necessitating further research to clarify the underlying mechanisms. Increased rates of the disorder are also found in people who were born prematurely, as well as in people who did not breastfeed in their newborn age. There are also studies linking ADHD to taking certain antibiotics at a young age [200,201,202,203]. However, these studies are not proof of the relationship, as there is a large degree of discrepancy between them. Indeed, in a cohort study in twins suggests that there is no association between ADHD and ASD diagnoses and early-life antibiotic use when environmental and genetic family factors are considered. Finally, in a population-based cohort study, the results suggest that early life antibiotic exposure has minimal impact on the risk of ASD and/or ADHD. Thus, the risk increase should not postpone reasonable antibiotic use. What could be taken as a conclusion is that the age of taking antibiotics plays a role in the later onset of ADHD, with a greater burden at younger ages. All the conditions are also related to changes in GM, thus raising the question of the association of ADHD with these changes [204,205,206] (Table 1).

Of all the neurodevelopmental disorders, the appearance of ASD has been most extensively linked to the presence of changes in GM. Years of observations have linked gastrointestinal disorders to autism. More specifically, increased incidence rates of diarrheal stools, constipation, flatulence, etc. have been demonstrated. The link between autism and inflammatory bowel disease has also been described in these individuals, which is called “autistic enterocolitis” [207]. At the same time, disturbances in the permeability of the intestine have been observed in people with ASD, resulting in the increased absorption of pathogenic substances such as endotoxemia with neurotoxins. Changes in the composition and balance among the various microorganisms of the GM appear to be related to the ASD phenotype [208,209]. A series of studies and meta-analyses in animal models and in humans in recent years has tried to prove the existence of this relationship, with particularly encouraging results. Regarding the composition of the GM, studies have shown an increase in the populations of Bacillota phyla, compared to those of Bacteroidota phyla, with a simultaneous decrease in Alistipes, Bilophila, Prevotella, and Veillonella and a significant increase in Collinsella, Corynebacterium, and the lactobacillaceae family in individuals with ASD. An increase has also been observed in the microbial populations of Clostridium and Akkermansia species in children with ASD compared to normal individuals [210,211,212]. Also, according to one study, bacteria of the genus Desulfovibrio were detected in almost half of the subjects with ASD and almost none in the normal subjects. Although the existence and, even more so, the nature, of a causal relationship between changes in the GM and autism has not been proven with certainty, several theories have been proposed in this direction [213]. Most theories concerning environmental influences focus on the fetal and perinatal period. Infections during pregnancy, and mainly, febrile infections with antipyretics, have been described in the prenatal history of individuals with autism [214,215]. Also, caesarean birth has been confirmed in a percentage of around 12–13% of people with ASD. This mode of birth alters the microbial colonization of the newborn, as instead of the normal microorganism of the mother’s vagina, the newborn is colonized mainly with skin microbiota [216]. The mode of birth affects the newborn’s initial microbiota colonization. Babies born by caesarean section are primarily colonized with skin microbiota, which can lead to GM imbalances (dysbiosis). This dysbiosis may influence neurodevelopment and is associated with an increased risk of ASD. The altered microbiota can affect gut permeability, immune system development, and the production of microbial metabolites, potentially contributing to ASD symptoms. Further research is needed to understand these mechanisms fully. Reference is also made to the administration of antibiotic substances both to the mother during pregnancy and to the newborn in the perinatal period. Antibiotic use has been shown to alter the GM, but there is insufficient evidence to demonstrate the permanence of these changes [217]. Alterations in the GM in individuals with ASD may cause rivalries between different strains, resulting in a reduction in microorganism diversity and function in the GM. Various metabolites and/or conjugates/derivatives of the microorganisms may change their action with the change in diversity in the GM, or the GM may be weakened, resulting in the appearance of the corresponding symptoms [218,219]. For example, the Clostridia species produces a substance, namely, 3-(3-hydroxyphenyl)-3 hydroxypropionic acid, which is considered harmful, as it reduces the concentrations of catecholamines. This substance is found to be elevated in autism and is blamed for the appearance of symptoms such as stereotypies in animal research models. Studies linking autism to the GM have paved the way for therapeutic interventions aimed at the GM could possibly reduce autism symptoms [220]. Probiotics are live bacteria that are found in various foods and their intake has several positive effects on the body. Specific species of probiotics, such as Bacteroides fragilis, have been shown in animal studies to improve behavioral disturbances, stereotypies, and anxiety symptoms often seen in ASD. Similar results are obtained from studies also in animals with the administration of some species of the Lactobacillaceae family [221,222,223]. Prebiotics are non-digestible nutritional substances, which serve as “food” for the non-pathogenic strains of the GM. Administration of prebiotics to people with autism, usually in combination with probiotics, had positive effects on the symptoms of the disorder. Taking certain antibiotics, such as vancomycin and metronidazole, had similar results, probably due to the limitation of PathoBact in the intestinal tract [224,225]. A more ambitious approach to treating ASD through the GM is transfer therapy and the transplantation of fecal microorganism (fecal microbial transplant) [226,227,228]. Although numerous studies have suggested an association between alterations in the GM and the symptoms of ASD, there is still no conclusive evidence demonstrating a direct causal link in humans, so further research is required.