High-efficiency and multiplex adenine base editing in plants using new TadA variants

TadA9, a new variant of an adenosine base editor, achieves efficient A-to-G conversion in rice at previously uneditable sites.

• TadA8e outperforms two other variants, TadA8.17 and TadA8.20, in editing efficiency across multiple target sites in the rice genome.
• The incorporation of V82S/Q154R mutations into TadA8e led to the development of the new variant, TadA9.
• TadA9 shows compatibility with various CRISPR systems, achieving similar or improved editing performance across diverse PAM sites.
• TadA9 was utilized to introduce novel SNPs in four herbicide target genes in the rice cultivar Nangeng 46.
• The generation of novel adenosine base editors suggests potential advancements in plant gene editing and crop molecular breeding.

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