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Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori
Two hormone-activated gene regulators identified in silkworm larvae
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Abstract
The expression of two transcription factors, E75 and E(spl)mβ, increased 3-8 times following the addition of juvenile hormone I (JH I).
- Two transcription factors, E75 and E(spl)mβ, are likely components of the juvenile hormone signaling pathways in the silkworm, Bombyx mori.
- DNA microarray analysis indicated that JH I induces the expression of E75 and E(spl)mβ in specific silkworm cells.
- Real-time RT-PCR confirmed significant increases in the expression of four E75 isoforms after JH I treatment.
- The expression of E75 and E(spl)mβ was directly induced by JH, as shown by the lack of suppression from a protein synthesis inhibitor.
- Similar JH-induced expression was also detected in various other silkworm cell lines and in larval hemocytes.
- Topical application of a JH analog, fenoxycarb, resulted in a strong induction of E75A expression in larval hemocytes.
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