Gene editing in living body cells using base and prime techniques

Base editors and prime editors can effectively deliver precise DNA changes in animal models.

• Base editors can correct the four most common single-base substitutions without causing extensive unwanted changes.
• Prime editors can install any substitution, insertion, or deletion over a range of dozens of base pairs.
• These editing technologies show greater efficiency and fewer byproducts compared to traditional methods that create double-strand DNA breaks.
• Both viral and non-viral methods have been used to successfully deliver these genome editing tools in vivo.
• The review highlights examples of somatic cell editing and discusses future development prospects.

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