bioRxiv : the preprint server for biology

A controllable gene-silencing system to study essential genes in Pseudomonas aeruginosa

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

A CRISPR interference system was developed that enables precise repression of 16 essential genes in Pseudomonas aeruginosa.

  • The system uses a rhamnose-inducible promoter to regulate the expression of gene-specific components.
  • Rapid construction of genetically modified strains allows for the study of various essential genes across multiple pathways.
  • Observations included variations in growth rates, antibiotic susceptibility, and transcriptional changes among different strains.
  • The system may facilitate the generation of pooled mutant libraries for comprehensive gene vulnerability assessments.
  • Insights gained could guide future antibiotic discovery efforts targeting this pathogen.

Simplified

Full Text

Full text is available at the source.
View Full Text ↗

Related papers

Feb '26
A controllable gene-silencing system to study important genes in Pseudomonas aeruginosa
top 10% journal
journal article
Jan '18
A Strong CRISPR-Based System to Turn Off Genes in Pseudomonas Bacteria
top 20% journal
cited by 100 papers
research support, u.s. gov't, non-p.h.s.
Oct '21
Shape and Gene Activity Changes After Turning Off Essential Genes in E. coli Using CRISPRi
top 10% journal
cited by 60 papers
research support, n.i.h., extramural
Apr '19
Reversible Control of Gene Activity in Yersinia pestis Using an Improved CRISPR Interference Method
top 20% journal
cited by 16 papers
research support, non-u.s. gov't
Oct '25
Using Chromosome and Plasmid CRISPRi Systems to Turn Off Genes in Lactococcus lactis
top 20% journal
journal article
Jul '24
A targeted gene-silencing screen finds a blocker of a key bacterial protein that helps build new proteins
preprint
Oct '23
Fast testing method to find tomato plants resistant to bacterial speck disease
top 50% journal
video-audio media
Jan '20
A CRISPR system for fast and efficient gene silencing in Caulobacter crescentus
top 10% journal
cited by 34 papers
research support, n.i.h., extramural
Jul '23
A high-throughput method to map important and non-important gene interactions across the bacterial genome
cited by 7 papers
preprint
Mar '20
Using the Streptococcus mutans CRISPR-Cas9 System to Study Essential Genes
top 5% journal
cited by 60 papers
research support, n.i.h., extramural

what lands in your inbox each week:

  • 📚7 fresh studies
  • 📝plain-language summaries
  • ✅direct links to original studies
  • 🏅top journal indicators
  • 📅weekly delivery
  • 🧘‍♂️always free

Recent issues from the crispr gene editing brief

issue #43
A rat virus enzyme boosted gene editing efficiency by 1.88x at hard-to-edit DNA sites
2 days ago7 studies
issue #42
A single dose edited 49% of liver cells in mice — without a virus
1 week ago7 studies
issue #41
CRISPR gene therapy eliminates blood transfusions in 91% of β-thalassemia patients
2 weeks ago7 studies
issue #40
90% of CRISPR guide RNA modified with new chemistry maintains full gene editing power
3 weeks ago7 studies
issue #39
AI-designed gene editor cuts off-target mutations by 553-fold
4 weeks ago7 studies
issue #38
AI-redesigned gene editors boost efficiency 2.9-fold in mice
5 weeks ago7 studies
issue #36
Gold nanoparticles deliver CRISPR and 2.1 kb DNA templates into blood cells
7 weeks ago7 studies
issue #35
CRISPR-Cas12a can now be guided by DNA instead of RNA to target RNA molecules
8 weeks ago7 studies
issue #34
CRISPR tool inserts 26kb DNA sequences with 40% efficiency
9 weeks ago7 studies
issue #33
Base editing corrected 60% of liver cells in mice with rare genetic disease
10 weeks ago7 studies
explore the archive
See more issues from the crispr gene editing brief