Improving IscB to create tiny, efficient gene editing tools for animal cells and embryos

An average 7.5-fold increase in activity was observed with engineered IscB proteins.

• Three substitutions in IscB proteins led to a significant increase in genome editing activity.
• The eIscB-D variant achieved a maximum editing efficiency of 91.3%.
• An engineered ωRNA was developed, resulting in a 20% reduction in length with improved efficiency.
• The eIscB-D/eωRNA system exhibited a 20.2-fold activity increase compared to original IscB.
• eIscB-D demonstrated high activity in mouse cell lines and embryos, facilitating disease model generation.

Simplified