Designing a small IscB enzyme to improve precise DNA base editing with wider targeting ability

IminiCBE achieved an average editing efficiency of 67% in mammalian cells.

• IscB has a similar structure to Cas9 but is smaller, making it more suitable for delivery via adeno-associated virus.
• High-efficiency miniature base editors were developed by modifying OgeuIscB and its associated RNA.
• IminiABE demonstrated an average editing efficiency of 52% in mammalian cells.
• The addition of the non-specific DNA-binding protein Sso7d to IminiBEs increased editing efficiency by approximately two- to threefold at low-efficiency sites.
• IminiCBE and SIminiCBE can target a broader range of genomic sites than traditional IscB nickase by recognizing specific motifs.

Simplified