ACS synthetic biology

Increased Nickase Activity Improves Adenine Base Editing

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

TurboABE demonstrates enhanced editing efficiency on adenine target sites without increasing off-target effects.

  • TurboABE is a modified adenine base editor that utilizes a TurboCas9 nickase to enhance genome editing.
  • The technology shows improved editing rates at adenine bases that are typically difficult to edit.
  • Editing efficiency is amplified across various adenine target sequences.
  • No increase in off-target editing was observed in both DNA and RNA.

Simplified

Full Text

Full text is available at the source.
View Full Text ↗

Related papers

Jan '23
Producing and Purifying a CRISPR-Cas9 Tool That Changes DNA Bases
top 50% journal
cited by 1 paper
research support, non-u.s. gov't
Jan '22
PhieABEs: A highly efficient adenine base editing tool without PAM limits for targeting many sites in plants
top 5% journal
cited by 73 papers
research support, non-u.s. gov't
Nov '24
Creating Heritable A-to-G DNA Changes in Arabidopsis Plants Using Adenine Base Editing and a Modified Cas9
top 20% journal
journal article

What Lands in Your Inbox Each Week:

  • 📚7 fresh studies
  • 📝plain-language summaries
  • ✅direct links to original studies
  • 🏅top journal indicators
  • 📅weekly delivery
  • 🧘‍♂️always free

Recent issues from the crispr gene editing brief

issue #48
CRISPR base editors delivered to rat brains reduced Huntington's protein fragments and improved movement
2 days ago7 studies
issue #47
AI tool uses AlphaFold3 to pinpoint exactly which gene editor residues cause off-target cuts
1 week ago7 studies
issue #46
A new delivery trick pushed prime editing efficiency up to 2.9-fold without extra off-target edits
2 weeks ago7 studies
issue #45
Scientists built humanized antibody mice in 8 weeks instead of over a year
3 weeks ago7 studies
issue #44
Scientists mapped 11,692 gene knockdowns in stem cells — all in one atlas
4 weeks ago7 studies
issue #43
A rat virus enzyme boosted gene editing efficiency by 1.88x at hard-to-edit DNA sites
5 weeks ago7 studies
issue #42
A single dose edited 49% of liver cells in mice — without a virus
6 weeks ago7 studies
issue #41
CRISPR gene therapy eliminates blood transfusions in 91% of β-thalassemia patients
7 weeks ago7 studies
issue #40
90% of CRISPR guide RNA modified with new chemistry maintains full gene editing power
8 weeks ago7 studies
issue #39
AI-designed gene editor cuts off-target mutations by 553-fold
9 weeks ago7 studies
explore the archive
See more issues from the crispr gene editing brief