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Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
Producing and Purifying a CRISPR-Cas9 Tool That Changes DNA Bases
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Abstract
CRISPR-Cas9-guided adenine base editors (ABEs) can convert A-T base pairs to G-C base pairs in genomic DNA.
- The delivery of preassembled ABE proteins with guide RNAs may significantly reduce off-target effects compared to other methods.
- High purity and quantity of ABE-gRNA complexes are essential for effective gene editing using the ribonucleoprotein delivery method.
- The procedure for expressing and purifying ABEmax, a high-efficiency version of ABE, is outlined.
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