A Lentiviral Fluorescent Reporter to Study Circadian Rhythms in Single Cells

Fluorescent circadian reporters based on the murine gene enable visualization of circadian rhythms in single cells.

• Circadian rhythms are self-sustained, ~24-hour cycles in gene and protein expression regulated by a molecular clock.
• Current visualization methods for circadian rhythms at the single-cell level are limited by reliance on genomic engineering.
• The presented reporters generate oscillatory signals that are dependent on the functionality of the circadian clock.
• These reporters can measure parameters like circadian phase in various cell types.
• The delivery method via lentiviral transduction makes these tools accessible for diverse biological studies.

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