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Transcriptomic analysis of Litopenaeus vannamei hepatopancreas under cold stress in cold-tolerant and cold-sensitive cultivars
Gene activity in the shrimp's liver-like organ under cold stress in cold-tolerant and cold-sensitive types
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Abstract
A total of 21,026 differentially expressed genes were identified between cold-tolerant and cold-sensitive shrimp under varying temperatures.
- Transcriptomic analysis was conducted on two shrimp cultivars, cold-tolerant Guihai 2 and cold-sensitive Guihai 1.
- 84.5 Gb of sequencing data led to the assembly of 143,029 unigenes, with 34.08% annotated in the Nr database.
- Significant enrichment of KEGG pathways related to lysosome, sphingolipid metabolism, and nitrogen metabolism was observed in cold-tolerant GH2 under cold stress.
- Eight key differentially expressed genes were selected for further validation using quantitative real-time PCR.
- This research provides a comparison of gene expression changes in response to cold stress between the two shrimp cultivars.
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