LncRNA FER1L4 suppressed cancer cell growth and invasion in esophageal squamous cell carcinoma.

May 18, 2018European review for medical and pharmacological sciences

Long non-coding RNA FER1L4 reduces growth and spread of esophageal squamous cell cancer cells

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Abstract

FER1L4 was found to be lowly expressed in esophageal squamous cell carcinoma tissues compared to normal tissues.

Overexpression of FER1L4 may inhibit cell proliferation and invasion in ESCC cells.
Increased FER1L4 levels could promote apoptosis and alter the cell cycle distribution towards the G0/G1 phase.
Knockout of FER1L4 is associated with enhanced proliferation and invasion of ESCC cells.
Loss of FER1L4 may inhibit apoptosis and decrease the cell cycle distribution in the G0/G1 phase.
FER1L4 appears to function as a tumor suppressor gene in the context of ESCC.

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OBJECTIVE: To investigate the regulatory effect of long non-coding ribonucleic acid (lncRNA) FER1L4 on biological behaviors of esophageal squamous cell carcinoma (ESCC) cells, such as proliferation and invasion.

PATIENTS AND METHODS: The expressions of FER1L4 were detected in 42 pairs of ESCC tissues and corresponding para-carcinoma tissues and 5 kinds of ESCC cell lines via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Polyethyleneimine (PEI) and liposomes were used for FER1L4 expression or interference elimination assays, respectively. The proliferation and invasion of ESCC cells were detected via MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), apoptosis assay, cell cycle assay, and transwell chamber.

RESULTS: Results of qRT-PCR showed that, compared with that in normal tissues, FER1L4 was lowly expressed in ESCC tissues. Overexpression of FER1L4 could inhibit cell proliferation and invasion, promote apoptosis and increase the cell cycle distribution in G0/G1 phase. Knockout of FER1L4 could promote the proliferation and invasion of ESCC cells, inhibit apoptosis and decrease the cell cycle distribution in G0/G1 phase.

CONCLUSIONS: FER1L4 is involved in the occurrence and development of ESCC and plays a key role as a tumor suppressor gene in ESCC.

LncRNA FER1L4 suppressed cancer cell growth and invasion in esophageal squamous cell carcinoma.

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