RETRACTED ARTICLE: Silencing of Long Noncoding RNA SNHG6 Inhibits Esophageal Squamous Cell Carcinoma Progression via miR-186-5p/HIF1α Axis

Dec 20, 2019Digestive diseases and sciences

Blocking the RNA SNHG6 slows esophageal cancer growth through the miR-186-5p and HIF1α pathway

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Abstract

LncRNA SNHG6 is upregulated in esophageal squamous cell carcinoma (ESCC) tissues and cell lines.

In ESCC tissues, lncRNA SNHG6 and HIF1α levels are increased, while miR-186-5p levels are decreased.
Knockdown of lncRNA SNHG6 leads to reduced proliferation, migration, and invasion of ESCC cells.
A negative correlation exists between lncRNA SNHG6 and miR-186-5p expression in ESCC tissues.
A positive correlation is observed between lncRNA SNHG6 and HIF1α expression in ESCC tissues.
LncRNA SNHG6 functions as a decoy for miR-186-5p, enhancing the expression of HIF1α.
Restoring SNHG6 or HIF1α can counteract the inhibitory effects of miR-186-5p on ESCC cell behaviors.

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AIMS: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 6 (SNHG6) has been shown to be upregulated in esophageal squamous cell carcinoma (ESCC). However, its detailed function in ESCC remains unknown. We investigated its specific roles in ESCC cell proliferation, invasion, and migration.

METHODS: Gene expression was evaluated by quantitative reverse transcriptase polymerase chain reaction and western blot. The subcellular localization of lncRNA SNHG6 was determined using subcellular location assay. Luciferase reporter assay and RNA pull-down assay were applied to determine the interaction between lncRNA SNHG6, miR-186-5p, and hypoxia-inducible factor 1α (HIF1α). The cell proliferation, migration, and invasion abilities were evaluated by using cell count kit-8, colony formation assay, and transwell migration and invasion assays.

RESULTS: LncRNA SNHG6 and HIF1α were upregulated, while miR-186-5p was downregulated in ESCC tissues and cell lines. Knockdown of lncRNA SNHG6 inhibits ESCC cell proliferation, migration, and invasion. A negative correlation between lncRNA SNHG6 and miR-186-5p expression was found in ESCC tissues. Similarly, there is a positive correlation between lncRNA SNHG6 and HIF1α expression in ESCC tissues. Conversely, miR-186-5p expression was negatively correlated with HIF1α expression in ESCC tissues. Furthermore, lncRNA SNHG6 was identified as a decoy for miR-186-5p, thereby promoting the expression of miR-186-5p target HIF1α. More significantly, restoration of SNHG6 or HIF1α could reverse the inhibitory effect of miR-186-5p on ESCC cell proliferation, migration, and invasion.

CONCLUSIONS: Downregulation of SNHG6 inhibited the proliferation, migration, and invasion of ESCC cells through regulating miR-186-5p/HIF1α axis, providing a novel therapeutic target for ESCC.

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RETRACTED ARTICLE: Silencing of Long Noncoding RNA SNHG6 Inhibits Esophageal Squamous Cell Carcinoma Progression via miR-186-5p/HIF1α Axis

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