supplementation restored recognition memory in a rodent model of chronic .
Recognition memory decreased due to LPS insult but improved with Neurosyn240 supplementation.
Behavioral changes from Neurosyn240 were linked to shifts in gut microbiota composition.
Certain gut microbiota changes significantly correlated with improved recognition memory performance.
Proteomic analysis revealed that both LPS insult and Neurosyn240 supplementation altered brain protein profiles.
Key pathways modulated included those related to energy production and neurodegenerative diseases.
Simplified
The Mediterranean diet (MD) and its bioactive constituents have been advocated for their neuroprotective properties along with their capacity to affect gut microbiota speciation and metabolism. Mediated through the gut brain axis, this modulation of the microbiota may partly contribute to the neuroprotective properties of the MD. To explore this potential interaction, we evaluated the neuroprotective properties of a novel bioactive blend () resembling the Mediterranean diet in a rodent model of chronic . Behavioral tests of cognition, brain proteomic analysis, 16S rRNA sequencing, andH NMR metabolomic analyses were employed to develop an understanding of the gut-brain axis interactions involved. Recognition memory, as assessed by the novel object recognition task (NOR), decreased in response to LPS insult and was restored with Neurosyn240 supplementation. Although the open field task performance did not reach significance, it correlated with NOR performance indicating an element of anxiety related to this cognitive change. Behavioral changes associated with Neurosyn240 were accompanied by a shift in the microbiota composition which included the restoration of theratio and an increase in, and most notablywhich significantly correlated with NOR performance.also correlated with the metabolites 5-aminovalerate, threonine, valine, uridine monophosphate, and adenosine monophosphate, which in turn significantly correlated with NOR performance. The proteomic profile within the brain was dramatically influenced by both interventions, with KEGG analysis highlighting oxidative phosphorylation and neurodegenerative disease-related pathways to be modulated. Intriguingly, a subset of these proteomic changes simultaneously correlated withabundance and predominantly related to oxidative phosphorylation, perhaps alluding to a protective gut-brain axis interaction. Collectively, our results suggest that the bioactive blend Neurosyn240 conferred cognitive and microbiota resilience in response to the deleterious effects of low-grade inflammation. 1Firmicutes: Bacteroidota Muribaculum, Rikenellaceae Alloprevotella Akkermansia Akkermansia Akkermansia
Key numbers
6% increase
Increase in Recognition Memory Performance
group vs. LPS group in NOR task performance
1.5×
Firmicutes to Bacteroidota Ratio Restoration
treatment restored this ratio compared to LPS insult
61 proteins
Significant Proteins Altered
Proteomic analysis linked to Akkermansia abundance
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DV received funding from Activ’Inside. L.P, D.G. work for Activ’Inside and provided the Neurosyn240 extract. Activ’Inside was not involved in the design, implementation, analysis, and interpretation of the data. All the other authors have no conflict of interest to declare.
