The Expression of the Melatonin Synthesis Enzyme: ArylalkylamineN-Acetyltransferase in the Suprachiasmatic Nucleus of Rat Brain

May 18, 1999Biochemical and biophysical research communications

Levels of the enzyme that makes melatonin in the brain’s daily rhythm control center of rats

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Abstract

AA-NAT enzyme activity in the rat suprachiasmatic nucleus is highest at early night and lowest at midday.

  • The serotonin N-acetyltransferase (AA-NAT) mRNA was found in the rat suprachiasmatic nucleus, which regulates biological rhythms.
  • AA-NAT mRNA levels did not exhibit a significant circadian rhythm.
  • Enzyme activity for AA-NAT showed a clear daily rhythm, peaking at night and dropping during midday.
  • The observed rhythm in AA-NAT enzyme activity persisted even in constant dark conditions.
  • These findings suggest that melatonin synthesis in the rat suprachiasmatic nucleus may be regulated by the circadian clock at a post-transcriptional level.

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