The Localization of the Site of ArylalkylamineN-Acetyltransferase Circadian Expression in the Photoreceptor Cells of Mammalian Retina

Jul 24, 1998Biochemical and biophysical research communications

Where the daily cycle of a key enzyme happens in light-sensing cells of the mammal eye

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Abstract

AA-NAT mRNA levels in the rat retina increased more than threefold at night.

  • AA-NAT is the rate-limiting enzyme for melatonin synthesis in mammals.
  • mRNA for AA-NAT was found exclusively in the retinal photoreceptors.
  • AA-NAT expression was low during the day and significantly higher at night.
  • The nocturnal expression of AA-NAT was confirmed through additional techniques.
  • This finding is the first to pinpoint the location of circadian AA-NAT expression in mammalian photoreceptor cells.

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