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Molecular Cloning of Crustacean Red Pigment Concentrating Hormone Precursor
Identifying the genetic code for the red pigment concentrating hormone in crustaceans
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Abstract
A cDNA encoding the precursor of the red pigment concentrating hormone (RPCH) was isolated from the shore crab, Carcinus maenas.
- The preprohormone sequence includes a 25 amino acid signal peptide and an eight amino acid RPCH sequence.
- A Gly acts as an amide donor in the precursor structure.
- The sequence also contains a dibasic processing site and a 74 residue peptide of unknown function, termed RPCH-precursor-related peptide (RPRP).
- In situ hybridization showed the RPCH gene transcript localized in two clusters of neurons in the medulla terminalis of the eyestalk.
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