Full text is available at the source.
Amplification-free detection of mycoplasma pneumoniae via CRISPR-Cas12a and deep learning-optimized crRNAs on a lateral flow platform
Direct detection of Mycoplasma pneumoniae using CRISPR-Cas12a and AI-optimized guides on a paper strip test
AI simplified
Abstract
The multiplexed crRNA library improved sensitivity by 16.8-fold, achieving a limit of detection of 0.15 pM.
- An amplification-free CRISPR-Cas12a detection platform was developed for Mycoplasma pneumoniae infection.
- The system uses a deep learning-optimized crRNA library targeting conserved regions of the MP P1 gene.
- Combinatorial use of 16 highly active crRNAs enhanced fluorescence signal intensity and reaction kinetics.
- The assay demonstrated a tenfold increase in visual detection sensitivity when deployed on lateral flow strips.
- Clinical evaluations showed over 95% agreement with standard clinical results and high specificity, with no cross-reactivity to other pathogens.
AI simplified