Neuropeptide‐mediated calcium signaling in the suprachiasmatic nucleus network

Nov 3, 2010The European journal of neuroscience

Neuropeptides and calcium signals in the brain’s internal clock network

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Abstract

VIP reduced the intracellular calcium concentration ([Ca(2+) ](i) ) in populations of SCN neurons during the day.

Neuroactive peptides and calcium levels are linked to gene expression modulation in SCN neurons, which influence behavioral rhythms.
AVP increased [Ca(2+) ](i) during both day and night, indicating a consistent effect regardless of neuronal firing activity.
PACAP responses varied among SCN neurons, showing no clear pattern of calcium signaling.
OFQ similarly reduced [Ca(2+) ](i) like VIP, suggesting a potential common mechanism.
The findings imply that VIP and AVP may play significant roles in regulating calcium balance in SCN neurons.

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Neuroactive peptides and the intracellular calcium concentration ([Ca(2+) ](i) ) play important roles in light-induced modulation of gene expression in the suprachiasmatic nucleus (SCN) neurons that ultimately control behavioral rhythms. Vasoactive intestinal peptide (VIP) and arginine vasopressin (AVP) are expressed rhythmically within populations of SCN neurons. Pituitary adenylate cyclase-activating peptide (PACAP) is released from retinohypothalamic tract (RHT) terminals synapsing on SCN neurons. Nociceptin/orphanin FQ (OFQ) receptors are functionally expressed in the SCN. We examined the role of several neuropeptides on Ca(2+) signaling, simultaneously imaging multiple neurons within the SCN neural network. VIP reduced the [Ca(2+) ](i) in populations of SCN neurons during the day, but had little effect at night. Stimulation of the RHT at frequencies that simulate light input signaling evoked transient [Ca(2+) ](i) elevations that were not altered by VIP. AVP elevated the [Ca(2+) ](i) during both the day and night, PACAP produced variable responses, and OFQ induced a reduction in the [Ca(2+) ](i) similar to VIP. During the day, VIP lowered the [Ca(2+) ](i) to near nighttime levels, while AVP elevated [Ca(2+) ](i) during both the day and night, suggesting that the VIP effects on [Ca(2+) ](i) were dependent, and the AVP effects independent of the action potential firing activity state of the neuron. We hypothesize that VIP and AVP regulate, at least in part, Ca(2+) homeostasis in SCN neurons and may be a major point of regulation for SCN neuronal synchronization.

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Neuropeptide‐mediated calcium signaling in the suprachiasmatic nucleus network

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