Purifying guide RNA for gene editing without chromatography

The non-chromatographic method for RNA purification achieves over 80% purity, meeting FDA standards for gene editing applications.

• A new purification method for synthetic RNAs simplifies the process by eliminating the need for chromatography.
• The method consists of five steps and is designed to enhance efficiency in RNA synthesis.
• Guide RNA targeting the GFP gene was successfully synthesized using this purification approach.
• Purity of the synthesized RNA was confirmed through analytical testing.
• Functional tests in solution and live mammalian cells demonstrate the RNA's effectiveness in CRISPR-Cas12a applications.

Simplified