Nutrient-induced glucagon like peptide-1 release is modulated by serotonin

May 5, 2016The Journal of nutritional biochemistry

Serotonin influences nutrient-triggered release of the hormone GLP-1

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Abstract

Fluoxetine increased nutrient-induced GLP-1 release from isolated pig intestinal segments.

Nutrient interaction with specific receptors stimulates GLP-1 release from enteroendocrine cells.
Casein and safflower oil were effective in stimulating GLP-1 release, while sucrose and rebaudioside A were not.
Fluoxetine, a serotonin reuptake inhibitor, further enhanced nutrient-induced GLP-1, PYY, and CCK release.
Serotonin release was stimulated by casein and safflower oil but not by sucrose or rebaudioside A.
Direct exposure to serotonin increased GLP-1 release from ileal tissue segments but did not affect PYY and CCK release.
Serotonin also stimulated GLP-1 release from STC-1 cells, which was inhibited by a non-specific serotonin receptor antagonist.

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Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists. Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50mM) and rebaudioside A (12.5mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release. Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155μM) further enhanced casein and safflower oil induced-serotonin release. Exposure of ileal tissue segments to serotonin (30μM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100μM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10μM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process.

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Nutrient-induced glucagon like peptide-1 release is modulated by serotonin

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