Recording sequences of cell events directly into DNA over time

peCHYRON enables high-information, durable recording of multiple cellular signals in mammalian cells.

• Genetically encoded DNA recorders can convert transient biological events into permanent mutations.
• The system utilizes a prime editor, a fusion protein that facilitates the insertion of variable DNA sequences.
• Insertions occur in a specific order, allowing for continuous editing and accumulation of information.
• Unique triplet DNA sequences from various guide RNAs enhance the amount of recorded information.
• Constitutive pegRNA expression enables the reconstruction of cell lineage relationships.
• Inducible pegRNA expression allows for precise recording of cellular responses to biological stimuli.

Simplified