Light-Activated One-Step DNA Test to Detect a Critically Endangered Species in the Environment

The limit of detection for the adapted assay is 6 copies/reaction.

• A novel assay was developed for on-site detection of environmental DNA (eDNA) from endangered species, specifically Bahaba taipingensis.
• The assay integrates isothermal amplification and a CRISPR-based detection method in a single tube, eliminating the need for thermal cycling.
• A portable fluorescence detector was combined with the assay to facilitate field deployment and immediate results.
• This approach may enhance the monitoring of endangered species by providing a rapid and sensitive testing method.

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