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Identification of OS-2 MAP kinase-dependent genes induced in response to osmotic stress, antifungal agent fludioxonil, and heat shock in Neurospora crassa
Genes controlled by OS-2 MAP kinase activated by salt stress, antifungal fludioxonil, and heat in Neurospora crassa
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Abstract
Expression of six genes related to glycerol synthesis, gluconeogenesis, and catalase was activated in response to osmotic stress in the wild-type strain, but not in the os-2 mutant.
- The two-component signal transduction system involving OS-1, OS-4, OS-5, and OS-2 is important for osmotic regulation in Neurospora crassa.
- Quantitative real-time RT-PCR analysis identified genes regulated downstream of the OS-2 MAP kinase.
- The wild-type strain showed increased expression of genes related to glycerol synthesis and gluconeogenesis when exposed to osmotic stress and fludioxonil.
- A heat shock treatment similarly induced expression of these genes in the wild-type strain.
- Glycerol dehydrogenase activity increased in response to osmotic stress and fludioxonil in the wild-type strain, indicating a functional response to stress.
- OS-2 phosphorylation occurs due to the OS-1 cascade under low osmotic stress and fludioxonil, while non-OS-1 signaling can activate OS-2 in an OS-4-dependent manner under heat shock and higher osmotic stress.
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