Full text is available at the source.
ATF-1 transcription factor regulates the expression of ccg-1 and cat-1 genes in response to fludioxonil under OS-2 MAP kinase in Neurospora crassa
How the ATF-1 protein controls ccg-1 and cat-1 gene activity in Neurospora crassa when treated with fludioxonil through the OS-2 signaling pathway
AI simplified
Abstract
The Deltaatf-1 strain showed no enzyme activity of the large-subunit catalase CAT-1, indicating its production is largely dependent on ATF-1.
- Disruption of the atf-1 gene results in osmotic sensitivity and resistance to fludioxonil being absent in the Deltaatf-1 strain.
- Up-regulation of the catalase gene cat-1 and the clock-controlled gene ccg-1 by fludioxonil or NaCl treatment was significantly reduced in the Deltaatf-1 strain.
- ATF-1 likely binds to the promoters of cat-1 and ccg-1 via specific DNA sequences known as CRE motifs.
- Expression of certain clock-controlled genes was induced by fludioxonil in an OS-2-dependent manner, although some induction occurred in the Deltaatf-1 strain.
- The Deltaatf-1 strain produced perithecia and ascospores, but the ascospores were unable to germinate.
AI simplified