Enhanced phosphorylation of p53 by microRNA-26a leading to growth inhibition of pancreatic cancer

miR-26a treatment decreased cell proliferation by 80% in pancreatic cancer cells with mutant p53.

• Significant inhibition of colony formation and cell migration was observed following miR-26a expression.
• Cell-cycle analysis indicated a blockage at the G0/G1 phase.
• Enhanced retention of chemotherapy drugs and increased sensitivity to gemcitabine were noted.
• Mutant p53 was rapidly phosphorylated at Ser9 and Ser392, which may restore its normal function.
• Gene analysis revealed increased expression of transcripts that promote cell death and p53 activation, alongside reduced levels of cell-cycle progression genes.

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