The Phospho-Code Determining Circadian Feedback Loop Closure and Output in Neurospora

Mapping revealed 80 phosphosites on WC-1 and 15 on WC-2 involved in circadian regulation.

• Phosphorylation of WC-1 and WC-2 is linked to the regulation of circadian oscillators.
• A specific code of phosphorylation events on WC-1 and clusters on WC-2 influences circadian repression.
• The study identifies rhythmic binding of the White Collar complex to gene promoters as crucial for circadian output.
• Weak correlations and contradictions exist between heterodimer activity and phosphorylation, indicating gaps in understanding.
• This work enhances the mechanistic knowledge of negative feedback processes in circadian biology.

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