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Abstract
PACAP and VIP receptors are expressed in human breast tissue, with higher levels in cancerous tissue compared to normal tissue.
- The three subclasses of PACAP/VIP receptors (VPAC1, VPAC2, and PAC1) were identified in both normal and malignant breast tissue.
- mRNA analysis revealed various isoforms of PAC1 receptors due to alternative splicing.
- Immunohistochemistry showed that these receptors are present in epithelial cells and leukocytes within carcinomatous tissue.
- A weaker presence of PAC1 receptors was noted in normal tissue, while cancer tissue demonstrated a strong density of all receptor subclasses.
- The functional activity of PAC1, VPAC1, and VPAC2 receptors was confirmed through their coupling to adenylate cyclase stimulation.
- The findings suggest a potential role for PACAP and VIP in breast tumor progression, although further validation with more samples is needed.
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