Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR‐Cas13a

The detection limit of the enhanced Cas13a method for porcine reproductive and respiratory syndrome virus (PRRSV) is 172 copies/μl.

• The method combines the collateral cleavage activity of LwCas13a with recombinase polymerase amplification (RPA).
• It operates at a constant temperature of 37°C for isothermal detection.
• The enhanced detection method allows for real-time analysis or visual readout.
• No cross-reactions were observed with porcine circovirus 2, porcine parvovirus, classical swine fever virus, or pseudorabies virus.
• The method is effective for use with clinical samples.

AI simplified