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Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay
Fast detection of dangerous pig reproductive and respiratory virus using a glowing probe and steady-temperature DNA test
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Abstract
The detection limit of the novel assay for highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV) is 70 copies of RNA per reaction.
- A new assay was developed for the rapid detection of HP-PRRSV using a fluorescent probe-based method.
- The assay showed high specificity, with no cross-reaction observed with other related viruses.
- Evaluation of 125 clinical samples indicated a strong correlation with existing real-time RT-qPCR assays for HP-PRRSV detection.
- These findings suggest the assay may be effective for quick identification of HP-PRRSV in clinical settings.
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