Post-encapsulation methods for the preparation of mRNA-LNPs

Production of mRNA-loaded lipid nanoparticles (mRNA-LNPs) using alternative methods may reduce material waste associated with conventional microfluidic mixing.

• Post-encapsulation of mRNA into pre-formed vesicles (PFVs) can be achieved through either microfluidic or manual pipetting methods.
• The physicochemical properties and morphologies of mRNA-LNPs produced by these alternative methods are similar to those from conventional microfluidic protocols.
• In vitro and in vivo assessments indicate that mRNA-LNPs generated by the alternative methods show comparable transfection levels to those from traditional formulations.
• The versatility of the post-encapsulation methods supports their applicability to various lipids, mRNAs, and microfluidic systems.

AI simplified