Recent progress and treatment possibilities of prime editing for human diseases

Prime editing (PE) offers improved editing efficiency without the need for double-strand breaks in DNA.

• PE can target point mutations as well as insertions or deletions in genomic DNA.
• This approach is associated with fewer byproducts compared to traditional gene editing methods.
• A variety of online resources and tools have emerged to facilitate personalized pegRNA design and off-target site prediction.
• The review covers the innovations and strategies behind PE systems and their auxiliary design tools.
• Potential applications of PE in clinical settings are discussed, highlighting its future prospects.

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