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Abstract
Prime editing (PE) offers improved editing efficiency without the need for double-strand breaks in DNA.
- PE can target point mutations as well as insertions or deletions in genomic DNA.
- This approach is associated with fewer byproducts compared to traditional gene editing methods.
- A variety of online resources and tools have emerged to facilitate personalized pegRNA design and off-target site prediction.
- The review covers the innovations and strategies behind PE systems and their auxiliary design tools.
- Potential applications of PE in clinical settings are discussed, highlighting its future prospects.
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