Prime editing: New methods, improvements, and future uses

Prime editing is a programmable genome-editing platform that can achieve targeted substitutions, insertions, and deletions without introducing double-strand breaks.

• Recent insights into flap dynamics and repair pathways enhance understanding of prime editing mechanisms.
• New engineered systems, including PEmax and PASTE, demonstrate high efficiency in gene editing.
• Prime editing has potential applications in therapeutic gene correction, agricultural biotechnology, and functional genomics.
• Challenges such as delivery, chromatin context, and mismatch-repair variability may limit broader application.
• Comparative analysis with other genome-editing techniques reveals prime editing's unique advantages.

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