The effect of progesterone on oxytocin-stimulated intracellular mobilization of Ca2+ and prostaglandin E2 and F2α secretion from porcine myometrial cells

Sep 26, 2006Prostaglandins & other lipid mediators

Progesterone’s influence on oxytocin-triggered calcium movement and prostaglandin release in pig uterine muscle cells

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Abstract

Oxytocin (10(-7)M) increases intracellular calcium levels in myometrial cells from pigs, but this effect is delayed during pregnancy.

Myometrial cells produce more prostaglandin E2 than F2alpha, regardless of the reproductive state.
Oxytocin enhances prostaglandin E2 secretion after 6 and 12 hours during luteolysis and after 12 hours in pregnant females.
The influence of oxytocin on intracellular calcium levels is delayed during luteolysis and inhibited during pregnancy when progesterone (10(-5)M) is present.
Progesterone diminishes the stimulatory effect of oxytocin on prostaglandin secretion.

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UNLABELLED: Our past studies have shown that porcine myometrium produce prostaglandins (PG) during luteolysis and early pregnancy and that oxytocin (OT) and its receptor (OTr) support myometrial secretion of prostaglandins E2 and F2alpha (PGE2 and PGF2alpha) during luteolysis. This study investigates the role of intracellular Ca2+ [Ca2+]i as a mediator of OT effects on PG secretion from isolated myometrial cells in the presence or absence of progesterone (P4). Basal [Ca2+]i was similar in myometrial cells from cyclic and pregnant pigs (days 14-16). OT (10(-7)M) increased [Ca2+]i in myometrial cells of cyclic and pregnant pigs, although this effect was delayed in myometrium from pregnant females. After pre-incubation of the myocytes with P4 (10(-5)M) the influence of OT on [Ca2+]i)was delayed during luteolysis and inhibited during pregnancy. Myometrial cells in culture produce more PGE2 than PGF2alpha regardless of reproductive state of the female. OT (10(-7)M) increased PGE2 secretion after 6 and 12 h incubation for the tissue harvested during luteolysis and after 12 h incubation when myometrium from gravid females was used. In the presence of P4 (10(-5)M), the stimulatory effect of OT on PG secretion was diminished.

IN CONCLUSION: (1) porcine myometrial cells in culture secrete PG preferentially during early pregnancy and produce more PGE2 than PGF2alpha, (2) OT controls myometrial PGF2alpha secretion during luteolysis, (3) release of [Ca2+]i is associated with the influence of OT on PG secretion, and (4) the effects of OT on PG secretion and Ca2+ accumulation are delayed by P4 during luteolysis and completely inhibited by P4 during pregnancy.

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The effect of progesterone on oxytocin-stimulated intracellular mobilization of Ca2+ and prostaglandin E2 and F2α secretion from porcine myometrial cells

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