Soudni lekarstviNov 25, 2003

Psilocybin and psilocin amounts in different mushroom types

Psychedelic Science Weekly Brief ↗PubMed ↗

Updated Mar 1, 2026

Abstract

At least three species of Psilocybe contain high levels of psychoactive components.

Psilocin and psilocybin were quantified in several mushroom species found in the region.
The study identified Psilocybe semilanceata, Psilocybe bohemica, and Psilocybe arcana as having significant amounts of these compounds.
Other species like Panaeolus acuminatus, Inocybe haemacta, and Pluteus salicinus were also analyzed for their psilocin and psilocybin content.

Simplified

Psilocin and psilocybin are psychoactive components of mushrooms of the genus Psilocybe and many others (Panaeolus, Inocybe, Pluteus etc.). In our republic, several species of Psilocybe with a high content of these components can be found. In the present study, we give a semiquantitative content of psilocin and psilocybin in some of our mushrooms in dry substance (Psilocybe semilanceata, Psilocybe bohemica, Psilocybe arcana, Psilocybe cyanescens, Panaeolus acuminatus sensu Ricken, Inocybe haemacta and Pluteus salicinus). For quantification, the GC/MS instrumentation was applied. Psilocin and psilocybin were silylated by the derivatization agent N-methyl-N-trimet-hylsilyltrifluoroacetamide. As an internal standard, 5-methoxytryptamin was used. The results of this study prove the presence of at least three species of Psilocybe with a high content of psychoactive components growing in our republic: Psilocybe semilanceata, Psilocybe bohemica and Psilocybe arcana.

Psilocybin and psilocin amounts in different mushroom types

Abstract

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