significantly accelerated the healing of bacterial-infected diabetic wounds in a rat model.
PPNM exhibited multienzyme-like activities, including those similar to peroxidase and catalase.
PPNM-Gel demonstrated strong tissue adhesion, water absorption, biodegradability, and biocompatibility.
In vitro tests showed PPNM-Gel had significant antibacterial effects against E. coli and S. aureus.
In the diabetic rat model, PPNM-Gel promoted wound remodeling by reducing inflammation and enhancing collagen deposition.
Simplified
PURPOSE: Treatment of bacterial-infected diabetic wounds is still challenging due to the susceptibility to bacterial infection and oxidative stress. Therefore, a novel multifunctional platform was established for accelerating the healing of bacterial-infected diabetic wounds.
METHODS: PtPdNi trimetallic-doped MIL-88 (PPNM) was fabricated and anchored to polyvinyl alcohol/sodium alginate (PVA/SA) matrix for constructing PPNM-containing hydrogel (). The basic properties of PPNM were characterized by TEM, SEM, XPS, FT-IR spectra, and UV-vis spectra. Adhesive, swelling, and degradation properties of PPNM-Gel were also analyzed. In vitro biocompatibility of PPNM-Gel was determined using L929 and HUVEC-SV40 cells. In vitro antimicrobial effects of PPNM-Gel were evaluated withand. In vivo antibacterial and wound healing evaluation of PPNM-Gel was performed in the STZ-induced diabetic SD rats, which were established by intraperitoneal injection of STZ, followed by full-thickness skin wound preparation and bacterial infection. Histological and immunofluorescence staining were used to evaluate the status of epithelization, collagen deposition and inflammation response. E. coli S. aureus
RESULTS: PPNM exhibited multienzyme-like activity including POD-like, OXD-like, CAT-like, SOD-like, and NAD-like activities. PPNM-Gel demonstrated excellent tissue adhesive activity, water absorption ability, biodegradability, and biocompatibility. In vitro testing, PPNM-Gel showed obvious antibacterial ability againstandin a PPNM dose-dependent approach, and its antibacterial ability againstwas stronger than that against. In the STZ-induced diabetic SD rats, PPNM-Gel effectively accelerated wound remodeling by killing pathogenic bacteria, reducing inflammation, and promoting collagen deposition. E. coli S. aureus E. coli S. aureus
CONCLUSION: PPNM-Gel significantly accelerated the healing of bacterial-infected diabetic wounds in the rat model. Therefore, PPNM-Gel showed great promise for the treatment of diseases affected by bacterial infections and high ROS levels.
Key numbers
98.0%
Wound Repair Area
Wound repair area of PPNM10-Gel group on day 12.
52.6%
Bacterial Colony Reduction
Destruction of bacterial biofilms by PPNM10-Gel.
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