Quantitative changes in branchial carbonic anhydrase activity and expression in the euryhaline green crab,Carcinus maenas, in response to low salinity exposure

Jun 1, 2006Journal of experimental zoology. Part A, Comparative experimental biology

Changes in gill enzyme levels and activity in green crabs exposed to low salt water

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Abstract

Hemolymph osmolality stabilized at new values by 24 hours after green crabs were transferred from 32 ppt to 10 ppt salinity.

  • A large increase in carbonic anhydrase (CA) mRNA concentrations in the posterior gills was observed by 24 hours post-transfer, remaining elevated for 4 days.
  • CA activity started to increase 48 hours after transfer, continuing to rise through 7 days.
  • CA protein concentration increased by 5-fold in the posterior gills of crabs acclimated to 10 ppt salinity.
  • No changes in CA activity, mRNA expression, or CA protein concentrations were detected in the anterior gills.
  • The observed changes in mRNA levels were transient, whereas the synthesized CA protein persisted in the gills for an extended period.
  • In separate experiments, CA activity and mRNA concentrations increased with decreasing salinity, peaking at 15 ppt before decreasing at 10 ppt.

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