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Abstract
The evaluation of 11 commonly used housekeeping genes identified ACTB and RPL13A as the most stable reference genes for blood-based circadian studies.
- Gene expression stability was analyzed in blood samples collected every 6 hours over a 24-hour period from 40 adults with varying severity of obstructive sleep apnea and controls.
- Commonly used housekeeping genes may show circadian oscillations, complicating the analysis of rhythmic gene expression.
- ACTB and RPL13A exhibited minimal variability across different sampling times and disease groups, making them reliable reference genes.
- Personalized cosinor analysis of core clock genes demonstrated that proper normalization allows for the detection of circadian rhythms in clinical samples.
- Significant circadian oscillations were observed in at least one clock gene among the participants when optimal normalization was applied.
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