Retinoblastoma Binding Protein 4 may affect how glioblastoma responds to temozolomide by controlling DNA repair proteins

RBBP4 modulates temozolomide (TMZ) sensitivity by regulating key DNA repair genes.

• Disruption of RBBP4 increased sensitivity to TMZ and induced synthetic lethality to PARP inhibition.
• RBBP4 silencing led to enhanced DNA damage signaling, increased H2AX phosphorylation, and apoptosis in GBM cells.
• Knockdown of RBBP4 reduced the expression of the DNA repair genes MGMT and RAD51.
• RBBP4 knockdown was associated with decreased acetylation and increased tri-methylation of histone H3 at specific gene promoters.
• RBBP4 is part of a chromatin-modifying complex with CBP/p300 that may regulate important genes involved in tumorigenesis.

Simplified