Activation of Rev-erbα attenuates lipopolysaccharide-induced inflammatory reactions in human endometrial stroma cells via suppressing TLR4-regulated NF-κB activation

Aug 15, 2019Acta biochimica et biophysica Sinica

Activating Rev-erbα reduces inflammation caused by bacterial toxins in human uterine support cells by blocking a key immune signaling pathway

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Abstract

LPS treatment increased mRNA levels of pro-inflammatory cytokines in human endometrial stroma cells (hESCs).

  • Rev-erbα plays a key role in regulating the balance between pro-inflammatory and anti-inflammatory responses in cells.
  • Pharmacological activation of Rev-erbα using SR9009 significantly reduced the production of pro-inflammatory cytokines in hESCs treated with LPS.
  • Knockdown of Rev-erbα led to increased levels of IL-1β, IL-6, and IL-8, along with elevated expression of the core clock gene Bmal1.
  • SR9009 inhibited the expression of toll-like receptor 4 (TLR4) and the activation of NF-κB, which were induced by LPS treatment.
  • These findings suggest that activating Rev-erbα may help mitigate inflammation in hESCs.

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