Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Clock gene expression is attenuated during decidualization in the rat uterus.

• Transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) decreased at implantation sites on day 6.5 compared to day 4.5.
• Ptgs2 transcripts increased on day 6.5, indicating a potential link between clock gene expression and decidualization.
• Immunohistochemistry confirmed similar changes in Rev-erbα and Ptgs2 in the endometrium on day 6.5.
• Decidual cells showed reduced rhythmic expression of clock genes alongside increased Ptgs2 transcription when induced by specific treatments.
• Rev-erbα antagonism led to increased Ptgs2 transcript levels and prostaglandin E₂ production.
• Rev-erbα directly binds to a regulatory site of Ptgs2, suggesting its role in modulating Ptgs2 expression during decidualization.

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