REV-ERBα Inhibits the PTGS2 Expression in Bovine Uterus Endometrium Stromal and Epithelial Cells Exposed to Ovarian Steroids

Jul 11, 2014The Journal of reproduction and development

REV-ERBα reduces PTGS2 levels in cow uterine support and lining cells exposed to ovarian hormones

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Abstract

The expression of REV-ERBα is significantly altered in bovine uterine stromal and epithelial cells exposed to ovarian steroids.

  • Clock genes in bovine uterine cells exhibit weaker circadian oscillation compared to those in rodents.
  • Prostaglandin F2α (PGF2α) secretion from uterine stromal cells is linked to the expression of the PTGS2 gene.
  • Treatment with progesterone suppresses PTGS2 expression in uterine stromal cells, whereas estradiol initially suppresses and then increases PTGS2 expression in epithelial cells.
  • Knockdown of BMAL1 leads to decreased levels of NR1D1 and PTGS2 transcripts in uterine stromal cells, but not in epithelial cells.
  • REV-ERBα antagonists increase PTGS2 expression in both cell types, while agonists have no effect.

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