Daily rhythms of REV-ERBα and its role as transcriptional repressor of clock genes in fish hepatic oscillator

Jun 8, 2023Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Daily cycles of REV-ERBα and its role in turning off clock genes in the fish liver's internal clock

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Abstract

A 12-hour shift in feeding time induced a parallel shift in the hepatic rhythm of rev-erbα expression in goldfish.

  • REV-ERBα is food-entrainable in the goldfish liver.
  • Light primarily drives rev-erbα rhythmic expression in the hypothalamus.
  • Activation of REV-ERBα led to a slight decrease in locomotor activity before light onset and food arrival.
  • REV-ERBα activation downregulated hepatic expression of key clock genes, including bmal1a and clock1a.
  • The repressive effect of REV-ERBα on hepatic clock gene expression was confirmed in vitro using specific agonists and antagonists.

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