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Rhythmic E-Box Binding by CLK-CYC Controls Daily Cycles in per and tim Transcription and Chromatin Modifications
Daily cycles of per and tim gene activity and DNA packaging controlled by CLK-CYC binding rhythm
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Abstract
Rhythmic transcription of the per and tim genes involves time-of-day-specific binding of CLK-CYC and associated chromatin modifications.
- Activation of per and tim transcription occurs alongside CLK-CYC binding to E-boxes and specific histone modifications.
- Histone H3-K9 acetylation and H3-K4 trimethylation are observed during the activation phase of these genes.
- RNA polymerase II binding to the tim promoter is linked to transcriptional elongation, while it remains constantly bound to the per promoter.
- Repression of transcription is associated with the reversal of these modifications by PER.
- Similar rhythmic histone modifications are linked to CLOCK-BMAL1-dependent transcription in mammals, suggesting a conserved mechanism across species.
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