Phosphorylation of the Transcription Activator CLOCK Regulates Progression through a ∼24-h Feedback Loop to Influence the Circadian Period in Drosophila

May 30, 2014The Journal of biological chemistry

Phosphorylation of the CLOCK protein controls the 24-hour biological clock cycle in fruit flies

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Abstract

Eliminating phosphorylation at four specific CLK sites shortens the circadian period in Drosophila.

  • Circadian clocks regulate daily rhythms in various organisms through transcriptional feedback loops.
  • CLK-CYC initiates the transcription of the period and timeless genes, which in turn regulate CLK-CYC activity.
  • Phosphorylation of CLK at specific sites is linked to its activity, influencing the timing of the feedback loop.
  • Identifying eight CLK phosphorylation sites reveals their role in modulating behavioral and molecular rhythms.
  • Loss of phosphorylation at certain sites can either accelerate the feedback loop or increase CLK activity.

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