How the Cas13b-ADAR2 complex guides targeted RNA editing

Cryo-electron microscopy structures of the Cas13b complexes reveal critical insights into RNA processing.

• The structures include the PspCas13b-guide RNA binary complex, the PspCas13b-guide RNA-target RNA ternary complex, and the dPspCas13b-ADAR2-guide RNA-target RNA complex.
• These structures provide mechanistic insights into how RNA is cleaved and edited.
• Engineering of a compact and efficient dPspCas13b-ADAR2 complex, termed REPAIR-mini, was achieved.
• Findings may enhance the understanding of CRISPR-Cas13 effector nucleases.
• This research could facilitate the development of advanced RNA-targeting technologies.

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