Diagnostic microbiology and infectious disease

Improved stability of a DNA-based test for respiratory germs using a special sugar and protective agent

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

The study aims to develop a composite lyoprotectant to enhance the stability of the RPA-CRISPR-Cas12a system for respiratory pathogen detection.

  • Acute respiratory infections caused by bacterial and viral pathogens represent a significant global health challenge.
  • Current point-of-care testing using the RPA-CRISPR-Cas12a system is limited by the instability of its lyophilized reagents.
  • Improving the stability of these reagents could facilitate the field deployment of rapid diagnostic tests for respiratory pathogens.

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Funding

Competing interests

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PubMed

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