Journal of microbiology (Seoul, Korea)Nov 27, 2025

Rapid and specific detection of Bandavirus dabieense using a combined RT-LAMP and CRISPR test

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Jan 3, 2026

Abstract

The optimized assay for Bandavirus dabieense detection demonstrated a detection limit of 5 RNA copies per reaction.

The assay combines reverse transcription loop-mediated isothermal amplification (RT-LAMP) with CRISPR/Cas12a technology.
LAMP primers and guide RNA were designed to target the L gene, allowing detection across various viral genotypes.
The assay showed more sensitivity than conventional qRT-PCR methods.
It exhibited 100% concordance with qRT-PCR results in clinical samples.
This tool may be suitable for rapid detection of SFTSV in resource-limited settings.

Simplified

Bandavirus dabieense, a single-stranded RNA virus, is the causative agent of severe fever with thrombocytopenia syndrome (SFTS), a disease associated with high fatality rates. Early and accurate diagnosis is essential for improving clinical outcomes, particularly given the limited therapeutic options and high mortality rates associated with SFTS. However, while highly sensitive, conventional diagnostic methods such as PCR and qRT-PCR require specialized laboratory facilities and trained personnel, making them impractical for rapid detection in resource-limited settings. To address these challenges, we developed a rapid and highly sensitive assay for Bandavirus dabieense detection by integrating reverse transcription loop-mediated isothermal amplification (RT-LAMP) with CRISPR/Cas12a technology. LAMP primers and guide RNA sequences were designed to target the L gene, ensuring broad detection across viral genotypes. The optimized assay demonstrated a detection limit of 5 RNA copies per reaction, showing more sensitivity than qRT-PCR, and exhibited 100% concordance with qRT-PCR results in clinical samples. Given its speed, accuracy, and field applicability, this LAMP-CRISPR/Cas12a-based assay represents a promising diagnostic tool for early SFTSV detection, particularly in resource-constrained environments where conventional molecular diagnostics are not readily available.